Anti-TRAF6 Antibody

Product Name

Anti-TRAF6 Antibody

View all TRAF-6 Antibodies

SKU/Catalog Number

A00185-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRAF6 Antibody (Catalog # A00185-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TRAF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TRAF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00185-1)

Host

Rabbit

Contents

TRAF6 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6. The immunogen is located within the last 50 amino acids of TRAF6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of IRF3 are known to exist.

Reactive Species

A00185-1 is reactive to TRAF6 in Human, Mouse, Rat

Applications

A00185-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

59.573kDa

Background of TRAF-6

Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.

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Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/mL.

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Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 μg/mL.

Gene/Protein Information For TRAF6 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase TRAF6; EC 6.3.2.-; Interleukin-1 signal transducer; RING finger protein 85; RNF85MGC:3310; TNF receptor-associated factor 6; TRAF6; TRAF-6 TRAF6 MGC:3310, RNF85 TNF receptor associated factor 6 TNF receptor-associated factor 6|E3 ubiquitin-protein ligase TRAF6|RING finger protein 85|RING-type E3 ubiquitin transferase TRAF6|TNF receptor-associated factor 6, E3 ubiquitin protein ligase|interleukin-1 signal transducer

For more info on TRAF6, check out the TRAF6 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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